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    A comparison of direct and calculated free 25(OH) Vitamin D levels in clinical populations.

    The authors compared direct quantitation of circulating free 25(OH) vitamin D (D) levels with calculated free 25 (OH) D levels and their relationships to iPTH, a biomarker of D effect, in humans.  They found that directly measured free 25-OH concentrations were related to iPTH but calculated estimates were not. Hence, they conclude that the current algorithms to calculate free 25-OH vitamin D may not be accurate.

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