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    Are osteoclasts needed for the bone anabolic response to PTH? A study of intermittent PTH With Denosumab or alendronate in knock-in mice expressing humanized RANKL

    J Biol Chem. 2010 Jun 17. [Epub ahead of print]

    In this study the ability of intermittent PTH to stimulate an anabolic bone response was tested in mice that were deficient in osteoclasts either because they were treated with an antibody to RANKL or because the mice were genetically deficient in RANK. It was found that there were both osteoclast-dependent and osteoclast-independent effects of intermittent PTH, which produced its anabolic response.

    Authors: Pierroz DD, Bonnet N, Baldock PA, et. al

    PTH stimulates osteoblastic cells to form new bone and to produce osteoblast-osteoclast coupling factors such as RANKL. Whether osteoclasts or their activity are needed for PTH anabolism remains uncertain. We treated ovariectomized huRANKL knock-in mice with a human RANKL inhibitor denosumab (DMAb), alendronate (Aln), or vehicle for 4 weeks, followed by co-treatment with intermittent PTH for 4 weeks. Loss of bone mass and microarchitecture was prevented by Aln and further significantly improved by DMAb. PTH improved bone mass, microstructure, and strength, and was additive to Aln but not to DMAb. Aln inhibited biochemical and histo-morphometrical indices of bone turnover, -i.e. osteocalcin and bone formation rate (BFR) on cancellous bone surfaces-, and Dmab inhibited them further. However Aln increased whereas Dmab suppressed osteoclast number and surfaces. PTH significantly increased osteocalcin and bone formation indices, in the absence or presence of either anti-resorptive, although BFR remained lower in presence of Dmab. To further evaluate PTH effects in the complete absence of osteoclasts, high dose PTH was administered to RANK-/- mice. PTH increased osteocalcin similarly in RANK-/- and WT mice. It also increased BMD in RANK-/- mice, although less than in WT. These results further indicate that osteoclasts are not strictly required for PTH anabolism, which presumably still occurs via stimulation of modeling-based bone formation. However the magnitude of PTH anabolic effects on the skeleton, in particular its additive effects with anti-resorptives, depends on the extent of the remodeling space, as determined by the number and activity of osteoclasts on bone surfaces.

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