•  
     
     
    Type Size
     

    Dynamic visualization of RANKL and Th17-mediated osteoclast function

    The authors visualized fluorescently labeled mature osteoclasts in intact mouse bone tissues using intravital multiphoton microscopy. Within this mature population, they found cells with static - bone resorptive (R) and moving - nonresorptive (N) characteristics. Rapid application of RANKL converted many N osteoclasts to R, suggesting a novel point of action in RANKL-mediated control of mature osteoclast function. Finally, they showed that Th17 cells, a subset of RANKL-expressing CD4+ T cells, could induce rapid N-to-R conversion of mature osteoclasts via cell-cell contact.