• May 19, 2015 | Webinar

    Defining Stages of Osteoprogenitor Lineage Differentiation

    Ivo Kalajzic, M.D., Ph.D.

    Studies on osteoprogenitor lineage differentiation rely on the use of the various bone markers that are related to the stage of differentiation. Use of stage specific expression of various mesenchymal/osteoprogenitor lineage promoters to drive the expression of green fluorescent proteins (GFPs) allows for in vivo and in vitro identification of cells at different stages of lineage differentiation. The main progress of this work is to define markers of preosteoblasts, osteoblasts and osteocytes, and characterizing the gene expression of the isolated cell populations.  Ongoing research by our and other groups has been focused on understanding the identity of mesenchymal progenitor cells (MPCs) in vivo.  This webinar will present attempts to identify, label and trace mesenchymal lineage cells into osteoblast lineage.

    Multiple approaches defined MPCs in vivo as perivascular population of cells within the bone marrow and labeled a mesenchymal progenitor cell within periosteum. This work was extended to understand the mechanisms that regulate commitment and osteogenic differentiation of these cells during bone fracture repair. The aim of this webinar is to provide a comprehensive view of the markers of mesenchymal progenitor cells, identify the stages along its differentiation pathway, and explain the approaches utilized to understand the mechanisms that regulate differentiation.

    Learning Objectives: 

    • Understand a visual approach to the identification of stages of cells at different maturational levels of osteoblast lineage.
    • Understand lineage tracing models that have been utilized to identify mesenchymal progenitor cells.
    • Discuss the approaches aimed to understand the mechanisms regulating mesenchymal progenitor cells commitment. 

    Length: 60 minutes

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